Free Radical OxiScore™ Pre-Test Protocols
OxiScore™ is a very sensitive colorimetric free radical test that can
detect minute amounts of MDA (malondialdehydes) in urine. Because OxiScore™ is so sensitive, it can be
affected by variables that may contribute to inconsistent readings. The detailed pre-test instructions
provided below will help limit these variables and enable you to produce consistent meaningful
results. Read these instructions thoroughly and follow them
carefully to produce a “Baseline Value” (initial starting point), and then in three to four
weeks retake the test to produce an “End Point Value”. The difference in your test results will be a
valuable tool to evaluate the progress of the nutritional and lifestyle changes you have
: Consult with your physician if you are
unsure of your ability to follow these instructions due to a pre-existing medical condition. OxiScore™ is
not designed to replace any medical tests or treatments. OxiScore™ is not intended to diagnose, treat or
prevent any disease.
INSTRUCTIONS: To be followed beginning 24 hours before taking the
OxiScore™ measures the concentration of MDA in urine. The concentration of MDA in urine is affected by the
amount of fluids one drinks the day before the test. For this reason fluid intake for the 24 hour period
prior to testing should be controlled and consistent each time the test is taken. Drinking the
recommended 8-10 glasses of water a day is advisable. Too little fluid intake will hyper-concentrate the
urine and can produce high readings. If you normally consume more water than this, or cannot consume the
recommended amount, then be sure to be consistent with how much you drink each time you take the test so that your
results will be consistent and comparable.
Caffeine is a type of drug called a diuretic. Diuretics cause increased fluid movement to the bladder and can
cause irregular voiding of urine. Caffeine, as a drug, can also increase free radical and MDA
Do Not Take Nutritional
Supplements, Vitamins or Antioxidants.
Any oral intake of vitamins or medication that
turns your urine to an excessively yellow color may interfere with your ability to interpret the reading of your
OxiScore™ test. Key vitamins that discolor urine are: vitamin C, vitamin B complex, vitamin B1 (thiamine),
vitamin B2 (riboflavin), vitamin B3 (niacin), vitamin B5 (panothenic acid), vitamin B6 (pyridoxine), vitamin B12
Alcohol usage of
any kind can increase test scores, including red wines.
Eat the Same
Dietary and nutritional intake is a key part of controlling oxidative stress. Certain foods can have strong
affects on MDA levels, especially if one experiences any types of reactions to the foods. We recommend having
something you enjoy eating regularly for breakfast lunch and dinner, and then to be sure to eat the same foods 24
hours before each time you test. The more consistent your life is 24 hours prior to the test, the more
meaningful the test results will be. You can log your food and liquid
intake on the chart provided - page 2 “Food and Liquid Intake Chart”.
tears down muscle tissue and that translates to high free radical and MDA levels in urine and a high OxiScore™
reading. However, MDA levels from strenuous exercise are NOT necessarily indicative of oxidative
stress. If possible, plan to take the test the day after your regular day of rest from
Use Consistent Times
Between Voids, and First Morning Voids.
MDA is continually collecting in the bladder 24 hours a day. OxiScore™ is measuring the amount of MDA
collected between the last time you voided your bladder and the present void. If there is a large discrepancy
between how many hours are spent between voids, this can translate into non-meaningful comparisons between
tests. 5-8 hours of collection time are recommended for the OxiScore™ test. There is a space at the
bottom of the dietary chart below to log void times and the total collection hours. The first morning
void is the recommended void to use with OxiScore™.
Food and Liquid Intake
Use this chart to log
your fluid intake and dietary choices for the 24 hours prior to taking the OxiScore™ test, as well as your last
void time and the number of hours between the last void time and the collection time. Try do duplicate these
choices each time you take the test. The more consistent you are with controlling these variables, the more
meaningful the results of the test will be. The Food and Liquid Intake chart is included in the Pretest
Protocols PDF link below.
Pretest Protocols (includes Food and Liquid Intake
Return from Free Radical
Pre-Test Protocols to Free Radical Test.