MALONDIALDEHYDE ( MDA ) MEASURED BY A FLUOROMETRIC AND VISUALLY READ COLORIMETRIC ASSAY IN
represents a paper that was presented at the Federated Experimental Biology and Medicine meetings
on April 15-18,
R. Hubbard, R. Iacono, J.
Westengard and T. Schoonenberg Depts. of Path., and Neurosurg. Sch. Med., Loma Linda
We have measured the urine Malondialdehyde ( MDA ) levels in
17 Parkinson disease (PD patients and care giver controls, by a fluorometric method (Conti et al., Clin. Chem.
37:1272, 1991) and a one step visually read colorimetric method adapted from Miksch et al., Anal Chem 53:2118,
1981, which is available through Vitaleaf Technologies, Irvine CA as OXISCORE. PD subjects and controls
volunteered for the study while attending the 1999 Iocono Pallidotomy Reunion. They collected random
midmorning urine's and signed consent forms approved by the Investigative Review Board, Loma Linda University
. To compare
the two methods a Spearman rank order correlation coefficient 0.656 (p<0.0005) was computed. A one-way
ANOVA with post hoc comparisons (Bonferroni) was performed to compare the mean fluorometric MDA levels across
the 4 semi-quantitative levels of the colorimetric method. The results showed that only the highest (level
compared to the lowest (level 0) were significantly different (p<0.02). Blind repetition of the
colorimetric assay showed a perfect match in the reading for 27 of the 34 urine specimens. The seven
non-matched results were within one level of matching. The difference in malondialdehyde levels between
patients and controls was significant when measured by either the fluorometric (independent t-test, p=0.00005)
or the colorimetric (Kruskal-Wallis, p=0.004) method. This abstract compares results determined with OXISCORE
with a very complex and sensitive laboratory assay for the same molecule namely malondialdehyde (MDA). MDA is
the first breakdown product from the action of "free radicals". We measure MDA in the urine because the MDA in
the blood is quickly cleared from the body by the kidneys and the urine becomes a body fluid where the MDA is
concentrated. The urine therefore is the ideal body fluid to measure MDA and thus a very sensitive way to look
for free radical activity. This study compares urinary MDA levels in Parkinson patients versus their
normal mates or companion care givers. The two assay methods give very comparable results. OXISCORE showed
that +3 results were significantly different from blank or zero values. Visual reading of the OXISCORE results
read against the test comparison strip were very repeatable in a blind test comparison showed better than 85%
correlation and no result was off by more than one level, e.g. +2 to a +3. The two assays gave very similar
significantly higher MDA results in the Parkinson patients versus the controls with the complex laboratory
assay giving somewhat more significant results.
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