Purpose of this study was to determine oxidative stress increase in aging. Increased MDA
levels was a key determining factor in support of the presence of increased oxidative stress in elderly
2. Mutlu-Turkoglu, Umit; Aykac-Toker, Gulcin; Ilhan, Ebru;
Kuru, Alev; Oztezcan, Serdar; Uysal, Mujdat
JOURNAL NAME- Clinical Biochemistry VOL. 36 NO. 5 July 2003 2003 PP. 397-400.
DOCUMENT TYPE- Article ISSN- 0009-9120
ADDRESS- Department of Biochemistry, Medical Faculty of Istanbul, University of Istanbul , Capa, 34093, Istanbul , Turkey , Turkey
Objectives: Increased oxidative stress has been hypothesized to play an important role in the aging process. A role
for oxidative damage in normal aging is supported by studies in experimental animals, but there is limited evidence
in humans. To investigate the relationship between the oxidative stress and aging in humans, we determined lipid
and protein oxidation in plasma as well as DNA damage in lymphocytes in young and elderly subjects. Design and
methods: 55 healthy subjects were divided into young (21-40 years) and elderly (61-85 years) groups. Plasma
malondialdehyde ( MDA ), protein carbonyl (PC) levels , and grade of DNA damage in lymphocytes using comet assay as
well as total ferric reducing antioxidant power (FRAP) in plasma were determined in young and elderly subjects.
Results: Plasma MDA and PC levels were found to be increased in plasma of elderly subjects as compared to young
subjects. Increases in endogenous and H2O2-induced DNA damage were also observed in lymphocytes of elderly
subjects. In addition, we detected a significant decrease in FRAP values in elderly subjects. Plasma MDA , PC
levels and endogenous and H2O2-induced DNA damage were positively correlated with aging, but negatively with FRAP
values. Conclusion: We evaluated MDA , PC levels and lymphocyte DNA damage altogether in both young and elderly
subjects for the first time. The results of this study strongly support the presence of increased oxidative stress
in elderly subjects.
Return from Free Radical Reference 2 Oxidative Stress
to Free Radical Laboratory Validation 1.